Standard staining

  • Chapter: Histology

    Article: 3 of 6

    Update: Feb 04, 2021

  • Author(s): Evert, Katja

  • Hematoxylin and eosin (H&E) stain: most important stain in histology; serves to differentiate between cell nuclei and cytoplasm (hematoxylin is the positively charged nuclear dye, basic; eosin is the negatively charged cytoplasmic dye, acidic)
  • Elastica van Gieson (EvG) staining: triple staining for differentiation of connective tissue and elastic tissue components (cell nuclei black-blue/black-brown, elastic fibers black-violet, collagen fibers red, muscles and cytoplasm yellow).